FAS Abstracts 2004 Meeting Page

Biological Sciences (BIO D): BIO-34

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Dose-dependent neurotoxicity of b-amyloid on septal glial cells.  B.R. GARCIA, A.J. SANCHEZ, and L.M. MUDD. School of Natural and Health Sciences, Barry University, 11300 NE 2^nd Avenue, Miami Shores, FL 33161. Alzheimer’s disease (AD) is characterized by senile plaques, which contain high levels of b-amyloid (BA) protein. The effect of dose dependent BA on septal glia was quantified by diamidino 2-phenyl indole (DAPI) fluorescence.  Glial cells previously cultured from embryonic day 16 rats were transferred onto silane-treated coverslips on day in vitro (DIV) 0.  The cells were subsequently treated with 0.1uM, 1uM, or 10uM BA or control on DIV 0, DIV 1, and DIV 2.  To quantify effects of BA, cells were counted on DIV 0, DIV 1, DIV 2 and DIV 3. 10uM BA appeared to have a toxic effect on septal glia.  This outcome, if significant, will indicate that glial cells may also be affected by BA in Alzheimer’s disease.  Supported by NIH MBRS grant # 45455 and MBRS Rise # IR25 GM9244.