FAS Abstracts 2004 Meeting Page
Medical Sciences (MED): MED-3
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Carbohydrate expression of the epithelial surface and the submucosal glands of asthmatic bronchi. M.D. CHUIDIAN (1), R.A. MIRZA (1), and A.T. MARIASSY (2). (1) Barry University, Miami Shores, FL 33161, (2) Nova Southeastern University, College Of Medical Sciences, Ft. Lauderdale, FL 33328. We hypothesize that the inflammatory disease which results in asthma elicits change for both submucosal secretions and the glycocalyx carbohydrate expression. We examined asthmatic airways with biotinylated lectins that recognize target carbohydrates: α-D-GalNAc, β-Gal(1-3)-D-GalNAc, GSA1, L-fucose, N-Acetylglucosamine, Gal (β,1-4)-GluNAc, D-Mannose, D-Glucose, N-acetylneuraminic a., L-fucose , (α,2-3) galactosamine, and α-sialyl. Lectin binding was detected with the Vector KitÒ ABC, avidin-biotin-HRP complex. RGB (red, green, blue) values were determined on digital images and compared by ANOVA. The micrographs depicted qualitative differences in glyco-conjugates of asthmatic bronchi.